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Novel Protein Precipitation Method for Whole Blood Using Automated Tip-on-Tip Technology

Novel Protein Precipitation Method for Whole Blood Using Automated Tip-on-Tip Technology

Purpose

  • Introduce innovative automated multi-purpose INTip filtration technology
  • Demonstrate the feasibility of a novel and rapid protein precipitation method, often referred to as “protein crash”.
  • This method can be extended to many areas of science, including drug discovery, metabolomics, and clinical trial studies.
  • This automated procedure could be applied to applications such as gelatin removal from drug formulations or processing cell lysates.

All sample preparation was performed on a Hamilton NIMBUS96 platform. 

This study shows that efficient protein precipitation using an automated liquid handler with simple pipetting commands can be substituted for laborious vortex mixing.  

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