The videos below highlight DPX methods and products. Click on each video to learn more.
DPX Blood Cleanup
This video demonstration shows a protein precipitated whole blood sample being aspirated and mixed inside a DPX-WAX tip (0.2 mL blood with 0.3 mL acetonitrile). After approximately 30 seconds, a clear and colorless (cleaned) solution is dispensed into a test tube and is ready for analysis. This rapid cleanup method produces a solution that is free of the ion suppression and matrix effects often encountered in LC-MS/MS analysis.
DPX Milk Cleanup
This video demonstration shows a rapid cleanup method for removal of proteins and sugars in a milk solution. Whole milk (100 uL) is pre-mixed with 50% acetonitrile and water (400 uL). The solution is then aspirated and mixed inside a DPX-WAX tip. The extraction requires only one minute to perform and produces a clear and colorless (cleaned) solution-ready for LC/MS or GC/MS analysis.
DPX-Qg for Removal of Chlorophyll
This video demonstration shows removal of chlorophyll from a spinach solution. Using the QuEChERS procedure, an acetonitrile spinach solution was prepared by shaking spinach with acetonitrile in a 1:1 ratio (10 g spinach with 10 mL acetonitrile). As shown, one test tube contains untreated acetonitrile solution (1 mL), while the second test tube contains acetonitrile solution (1 mL) that has been “cleaned” by aspirating and dispensing the solution in/out of the DPX-Qg tip, three times. Note the removal of chlorophyll (green pigment). DPX-Qg tips contain graphitized carbon black (GCB). This material is of the highest quality for removing plant pigment matrices that may be detrimental to a GC inlet system.
This video demonstration shows the DPX Extractor, a semi-autosampler that permits simultaneous extraction of 24 samples (1 mL tips) or a 96 deep well plate. The unit requires no electricity as extractions are performed using gas pressure (compressed air). It is easy to operate, robust and requires little to no maintenance. A second extractor is also available for simultaneous extraction of 48 samples (5 mL tips).
This video demonstration shows extraction of an aqueous mixture of two dyes (red and blue) using DPX tip technology. The blue dye is acidic, while the red dye is nonpolar. Extraction begins with a tip conditioning step, using methanol (middle test tube). Methanol is then dispensed and the sample solution is drawn into the DPX tip and mixed with air (left test tube). Following a short 30 second equilibration period, the solution is expelled. To enhance extraction efficiency, this step is repeated with another brief 10 second equilibration period. A colorless solution is dispensed from the DPX tip-indicating complete extraction of each dye from mixture.
In the final steps, elution of the dyes is accomplished with two solvents. The first solvent, methanol, elutes the acidic blue dye (middle test tube). The second solvent, methylene chloride, elutes the nonpolar red dye (right test tube). Each elution step requires only 10 second equilibration periods. Note that the solvent volumes required are significantly less than the sample solution volume, such that a built-in “concentration factor” is achieved.
Some DPX applications require no solvent evaporation or additional concentration steps-resulting in faster throughput!
Using a multi-channel pipettor, ion suppression effects are removed from protein precipitated serum, plasma or whole blood samples in just seconds. This video demonstration shows simultaneous cleanup of eight whole blood acetonitrile extracts using a multi-channel pipettor, 1 mL DPX tips, and a 96 deep well plate. In just 60 seconds, ion suppression and matrix effects are removed by aspirating and dispensing the solutions, three times.