Project Description

Automated Comprehensive Extraction of Drugs and Metabolites in Oral Fluid

Automation Compatibility: Hamilton and Integra

Oral fluid is gaining popularity as an alternative matrix for drug analysis. Oral fluid collection is simple, non-invasive, and can be easily monitored. Like other complex biological matrices, sample preparation is required to remove matrix interferents from oral fluid samples prior to LC-MS/MS analysis. This procedure can be time-consuming and is often the “bottle neck” for laboratory analysis and throughput.

Dispersive Pipette XTRaction methods require much less solvent and time-consuming steps compared to other SPE techniques. Automation of the extraction is easy to achieve with XTR tips for Hamilton or Integra liquid handlers. Automated Dispersive Pipette XTRaction is highly reproducible and provides the sensitivity necessary for forensic and clinical purposes.

1 ConditionUsing Mixed Mode WAX/RP- XTR tips Aspirate and Dispense Methanol
2 Bind AnalytesAspirate and Dispense oral fluid using WAX/RP- XTR tips
3 WashAspirate and Dispense Water
4 Elute AnalytesAspirate and Dispense Acetonitrile
5 Solvent Evaporation and ReconstituteCan be optimized based on necessary sensitivity.
XTR - Hamilton 300 μL CO-RE tips

XTR Tips for Hamilton

Tip Formats:

  • Hamilton CO-RE Technology – 300 μL and 1 mL

XTR tips for Hamilton can fit on any of the platforms for high throughput sample purification methods.

XTR Tips for Integra

Tip Formats:

  • 300 μL and 1250 μL


Morphine, Oxymorphone, hydromorphone, Codeine, Oxycodone, MDA, 6-MAM, Methamphetamine, Hydrocodone, MDMA, Norfentanyl, Tramadol, Normeperidine, Cocaethylene, Zolpidem, Cyclobenzaprine, Norbuprenorphine, Fentanyl, PCP, Buprenorphine, Oxazepam, Nortriptyline, Methadone, Nordiazepam, Alprazolam, Lorazepam, Amitriptyline, Temazepam, THC