Automated Comprehensive Extraction of Drugs and Metabolites in Oral Fluid
Automation Compatibility: Hamilton and Integra
Oral fluid is gaining popularity as an alternative matrix for drug analysis. Oral fluid collection is simple, non-invasive, and can be easily monitored. Like other complex biological matrices, sample preparation is required to remove matrix interferents from oral fluid samples prior to LC-MS/MS analysis. This procedure can be time-consuming and is often the “bottle neck” for laboratory analysis and throughput.
Dispersive Pipette XTRaction methods require much less solvent and time-consuming steps compared to other SPE techniques. Automation of the extraction is easy to achieve with XTR tips for Hamilton or Integra liquid handlers. Automated Dispersive Pipette XTRaction is highly reproducible and provides the sensitivity necessary for forensic and clinical purposes.
|1 Condition||Using Mixed Mode WAX/RP- XTR tips Aspirate and Dispense Methanol|
|2 Bind Analytes||Aspirate and Dispense oral fluid using WAX/RP- XTR tips|
|3 Wash||Aspirate and Dispense Water|
|4 Elute Analytes||Aspirate and Dispense Acetonitrile|
|5 Solvent Evaporation and Reconstitute||Can be optimized based on necessary sensitivity.|
XTR Tips for Hamilton
- Hamilton CO-RE Technology – 300 μL and 1 mL
XTR tips for Hamilton can fit on any of the platforms for high throughput sample purification methods.
XTR Tips for Integra
- 300 μL and 1250 μL
Morphine, Oxymorphone, hydromorphone, Codeine, Oxycodone, MDA, 6-MAM, Methamphetamine, Hydrocodone, MDMA, Norfentanyl, Tramadol, Normeperidine, Cocaethylene, Zolpidem, Cyclobenzaprine, Norbuprenorphine, Fentanyl, PCP, Buprenorphine, Oxazepam, Nortriptyline, Methadone, Nordiazepam, Alprazolam, Lorazepam, Amitriptyline, Temazepam, THC