Project Description

Quantitation of Fentanyl, Norfentanyl, Buprenorphine, and Norbuprenorphine in Urine using SCX-XTR tips and LC-MS/MS

Extraction 96 samples in < 15 minutes
Automation Compatibility: Hamilton

Fentanyl and buprenorphine are potent analgesics making them medicinally appealing and easy to abuse. Thus, they are two of the most common analytes monitored in clinical and forensic laboratories. Due to their high potency and low dosages, urinary concentrations are much lower than other common opiates/opioids. This leaves most laboratories struggling to increase the sensitivity and speed of their methods. This method using DPX INTip technology with strong cation exchange (SCX) sorbent promotes an easy, fast, and sensitive method to help laboratories increase efficiency of detecting these compounds and their metabolites.

Sample Preparation Workflow:

CONDITIONAspirate/ dispense 30% MeOH
BIND SAMPLEAspirate/ dispense using XTR tips with SCX sorbent x5
WASH 1Aspirate/ dispense 0.1 N HCl x2
WASH 2Aspirate/ dispense 100% MeOH x2
ELUTE ANALYTESAspirate/ dispense DCM/IPA/NH4OH x3

Download Application Note

XTR Tips

Product Information:

To order the exact product in this application use this catalog number.

Catalog: DPX170178              Description: XTR tips: 5 mg SCX (60 µm) in 1 mL Hamilton

To customize this product choose sorbent amount and tip format from the available options.