Quantitation of Fentanyl, Norfentanyl, Buprenorphine, and Norbuprenorphine in Urine using SCX-XTR tips and LC-MS/MS
Extraction 96 samples in < 15 minutes
Fentanyl and buprenorphine are potent analgesics making them medicinally appealing and easy to abuse. Thus, they are two of the most common analytes monitored in clinical and forensic laboratories. Due to their high potency and low dosages, urinary concentrations are much lower than other common opiates/opioids. This leaves most laboratories struggling to increase the sensitivity and speed of their methods. This method uses INTip Solid Phase Extraction. XTR tips with strong cation exchange (SCX) sorbent provide an easy, fast, and sensitive extraction method to help laboratories increase efficiency of detecting these compounds and their metabolites.
Sample Preparation Workflow
| CONDITION | Aspirate/ dispense 30% MeOH |
|---|---|
| BIND SAMPLE | Aspirate/ dispense using XTR tips with SCX sorbent x5 |
| WASH 1 | Aspirate/ dispense 0.1 N HCl x2 |
| WASH 2 | Aspirate/ dispense 100% MeOH x2 |
| ELUTE ANALYTES | Aspirate/ dispense DCM/IPA/NH4OH x3 |
Pre-Scripted Application for Hamilton Robotics
To order the exact product in this application and duplicate this method use this DPX catalog number.
| DPX Product | Sample Volume | Offline Processing | Online Processing |
|---|---|---|---|
| Catalog #: DPX170178 XTR tips with 5 mg SCX (60 µm) in 1 mL Hamilton | 100 µL | Solution Prep Solvent Evaporation | < 15 minutes |
To customize this product for a varying sample volumes, choose sorbent amount and tip format from available options.
