Proven Chemistry. Smarter Workflow for
High-throughput Genomic Sample Preparation

Simplify DNA extraction, cleanup, and size selection with silica-based functionalized pipette tips

NiXTips contain proprietary silica media to facilitate methods for DNA extraction from biological samples and methods for PCR cleanup or size selection.

Microporous Silica Membrane

Traditional chemistry inside a pipette tip!

NiXTipsMagnetic BeadsSpin Column
High Efficiency✅✖️✖️
Cost Effective✅✖️✅
Scalable✅✅✖️
Automation Compatible✅✅✖️
No Hardware✅✖️✖️
INTip Nucleic Acid Purification and Cleanup methods are powered by MicroPorous Xtraction (MPX) TechnologyHamilton style tip shown here

Pre-treatment

Wash 2

Elute

Wash 1

Bind

Add IPA

DNA binds to silica media

Extraction Workflow

Extraction Performance

High capacity silica media allows for concentration factors comparable to traditional spin column or magnetic bead workflows, but with a simple bind-wash-elute protocol.
Direct comparison of average DNA yields from 250 μL of blood with DPX method for isolation using 1 mL NiXTips versus the leading bead-based and column-based.

Example data for a 250 µL sample volume using a 1 mL NIXTip. NiX methods can support 50-500 µL sample volume.Comparison of gDNA yields (ng/μL or total ng) from 300 μL NiXTips vs. bead-based method across the 5 dilutions, based on qPCR data from QuantStudio 3.
Solid Phase Reversible Immobilization (SPRI) is commonly used for purifying and size selecting nucleic acids in Next Generation Sequencing (NGS) library preparation methods and a variety of applications for genomics research. NiXTips utilize a proprietary microporous media and buffer system to facilitate SPRI methods and allow for fast and effective removal of primers, primer-dimers, dNTPs, unincorporated labeled nucleotides, enzymes, and salts from PCR and other reaction mixtures.Application Notes:

Cleanup Workflow

PCR Cleanup and Size Selection Performance

Recovery of DNA MWM VIII in ng/μL with different (5, 10, 15, 20, 25, 30) aspirate/dispense cycles (binding cycles).Percent recovery of DNA fragments at different binding buffer to sample ratios.

How to Order

NiXTips are compatible with major liquid handling robotic platforms. NiXTips are sold in matrix-specific kits for DNA extraction and prep kits for cleanup methods, including necessary buffers. Download product specifications by clicking the applications available. Contact us for custom method development for applications not listed.

NiXTips Applications

Click the application links to download product specification sheets.

★  DNA Isolation from Cell Culture

★ DNA Isolation from Whole Blood

★ DNA Isolation from Saliva*

★ PCR Cleanup, Library Prep, Size Selection

*Saliva method coming soon

Hamilton format tips shown here. 

Tip Size -96 tips/rack Application
50 µL ★
300 µL ★  ★  ★
1 mL ★  ★  ★  
Tip Size Application
125 µL -384 tips/box  ★ ★
300 µL -96 tips/rack ★ ★ ★
1250 µL -96 tips/rack ★ ★ ★
Tip Size -384 tips/box Application
70 µL ★
Tip Size -96 tips/rack Application
200 µL ★  ★  ★

Tecan style NiXTips are coming soon!

Learn more

Evaluate NiXTips® for your genomic sample preparation.