INTip™ Filtration

INTip Filtration utilizes a patent-pending Tip-on-Tip technology designed to promote the simplification and automation of complex sample preparation for a variety of applications.

ToT Filtration

ToT Filtration combines a conductive wide bore top tip with a bottom Filtration tip to provide an automated alternative to traditional filtration/centrifugation. This methodology is ideal for high-throughput protein precipitation and filtration, beta-glucuronidase enzyme removal, or any other particulate removal, like cannabis solvent extract. Filtration tips can be ordered in low porosity, dual phase or single phase varieties.

Top- Wide Bore Conductive Tip

Filtration Tip

Gasket - provides air tight seal

Filter Options
Dual Phase - shown here
Low Porosity
Single Phase

Advantages:
  • Particulate removal down to less than 1 μm
  • No positive pressure/vacuum manifold
  • High throughput workflows that can process up to 96 samples in under 2 minutes
  • Low cost/ high efficiency solution

• ToT Filtration Anatomy and Advantages

Click the links to sort the slider.

• Simple Filtration Workflow

• Protein Precipitation Workflow

Aspirate Dirty Sample

ToT Simple Filtration

Tip-on-Tip (ToT)

Dispense Clean Sample

Particulate removal down to less than 1 μm

Tip-on-Tip automated protein precipitation workflow graphic

ToT Protein Precipitation

ToT Cleanup

Top- Wide Bore Conductive Tip

Single Phase Filtration Tip

Gasket - provides air tight seal

Loose sorbent
Customizable

Sorbent stays in Filtration Tip

Frit

This methodology is ideal for the cleanup of viscous matrices not compatible with XTR tips. ToT cleanup is compatible with a variety of sorbent chemistries to remove particulates and matrix interferents. The ToT Cleanup method can utilize single phase or low porosity filtration tips.

Advantages:
  • Uses Filtration tip + sorbent for cleanup
  • Cleanup for viscous matrices like cell lysates
  • Customer can provide their own sorbent
  • Compatible with a variety of sorbent chemistries
  • Easy to automate and easy to implement

• ToT Cleanup Method Workflow

• ToT Cleanup Anatomy and Advantages

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Aspirate Sample/Sorbent

Easy Sample Cleanup

Tip-on-Tip (ToT)

Analyze

Interferents Bind to Sorbent

Dispense Analyte of Interest

ToT SPE Anatomy

Top- Wide Bore Conductive Tip

Single Phase Filtration Tip with Disperser

Gasket - provides air tight seal

Disperser

Frit

ToT Solid Phase Extraction

Advantages:
  • SPE for viscous sample matrices
  • Customer can provide their own sorbent
  • Compatible with a variety of sorbent chemistries
  • Ideal for nanoparticle and antibody-conjugate drug formulation analyses

• ToT SPE Anatomy and Advantages

Click the links to sort the slider.

• ToT SPE Method Workflow

Tip-on-Tip Solid Phase Extraction provides a fast, automated INTip solution for a variety of applications including immunoaffinity. ToT SPE is ideal for applications that require specific incubation conditions between sorbent and sample solution.

ToT SPE utilizes a bottom Single Phase Filtration tip with a disperser. The disperser provides optimal dispersive solid phase extraction conditions. This tip has two frit options available: A low retention 50 μm frit and 30 μm frit.

Aspirate
Sample/Sorbent

Bind - Wash - Elute

Tip-on-Tip (ToT)

Sorbent stays in tip

Dispense

Wash

Elute

Analyze

Sample/Sorbent incubation can be performed in an automated fashion via aspiration/dispense steps, on a heater/shaker block, or under offline conditions

Download Product Ordering Guide

Introduction to ToT Technology

Videos

Products

The ToT product line is compatible with Hamilton Robotics systems. Filtration tips come in different varieties to support ToT methods for Filtration, Cleanup and SPE. DPX also offers a accessories to support adopting ToT methods.

Dual Phase Filtration Tips

Catalog Number: DPX170037

Two filters in series provide maximum filtration without clogging for higher volume blood and serum protein precipitation applications.

Compatible with all cannabis matrices (dried flower, edibles, extracts and concentrates) for pre-filtration prior to downstream analysis.

Low Porosity Filtration Tip <1 μm

Catalog Number: DPX170068

High efficiency sub-micron matrix filtration including urine, blood, and serum.

Compatible with all cannabis matrices (dried flower, edibles, extracts and concentrates) for pre-filtration prior to downstream analysis.


Catalog Number: DPX170068U

Low Porosity Filtration tips- Ultra Pure
Ultra pure grade for low detection level methods

Single Phase Filtration Tip

Catalog Number: DPX170069
  • This tip is compatible with Cleanup and SPE methods
  • Sorbent customization available
  • This tip is sold blank. Sorbent not included.

Minimal dead volume ideal for small volume blood and serum protein precipitation applications.

Compatible with sorbent particle sizes as low as 30 μm.

Single Phase Filtration Tip with a Disperser

  • Disperser allows optimal solid phase extraction conditions for SPE Methods
  • Sorbent customization available
  • This tip is sold blank. Sorbent not included.
  • Two frit options for variety of particle sizes
Catalog Number: DPX170097
Low retention frit promotes minimum dead volume and maximum flows. Ideal for sorbents greater than 50 μm in size.
Catalog Number: DPX170098
Ideal for sorbents in the 30-50 μm particle size range.

Sorbent Well Plate

SPS Format Plate Pre-loaded with sorbent for ToT SPE methods

Customize the sorbent mass available in a 96 deep well-plate for use with ToT SPE methods. These can contain any DPX sorbent in a range of masses per well. Contact us for sorbent options not listed.

  • WAX
  • WCX
  • SAX
  • SCX
  • RP
  • C18
  • Silica
  • Mixed Mode
  • HybridSPE® – Phospholipid removal

Interested in ToT Technology?

If you are unsure which Filtration Tip to request for your protocol, please consider filling out a product request form. Our team of application scientists will be able to better recommend a product or help you with custom method development with your sample matrix, volume and method parameters provided. We offer confidential method development and on-site technical support to help you get through the validation process.

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