INTip™ Filtration
INTip Filtration utilizes a patent-pending Tip-on-Tip technology designed to promote the simplification and automation of complex sample preparation for a variety of applications.
ToT Filtration
ToT Filtration combines a conductive wide bore top tip with a bottom Filtration tip to provide an automated alternative to traditional filtration/centrifugation. This methodology is ideal for high-throughput protein precipitation and filtration, beta-glucuronidase enzyme removal, or any other particulate removal, like cannabis solvent extract. Filtration tips can be ordered in low porosity, dual phase or single phase varieties.
Top- Wide Bore Conductive Tip
Filtration Tip
Gasket - provides air tight seal
Filter Options
Dual Phase - shown here
Low Porosity
Single Phase
- Particulate removal down to less than 1 μm
- No positive pressure/vacuum manifold
- High throughput workflows that can process up to 96 samples in under 2 minutes
- Low cost/ high efficiency solution
• ToT Filtration Anatomy and Advantages
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• Simple Filtration Workflow
• Protein Precipitation Workflow
ToT Cleanup
Top- Wide Bore Conductive Tip
Single Phase Filtration Tip
Gasket - provides air tight seal
Loose sorbent
Customizable
Sorbent stays in Filtration Tip
Frit
This methodology is ideal for the cleanup of viscous matrices not compatible with XTR tips. ToT cleanup is compatible with a variety of sorbent chemistries to remove particulates and matrix interferents. The ToT Cleanup method can utilize single phase or low porosity filtration tips.
- Uses Filtration tip + sorbent for cleanup
- Cleanup for viscous matrices like cell lysates
- Customer can provide their own sorbent
- Compatible with a variety of sorbent chemistries
- Easy to automate and easy to implement
• ToT Cleanup Method Workflow
• ToT Cleanup Anatomy and Advantages
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ToT SPE Anatomy
Top- Wide Bore Conductive Tip
Single Phase Filtration Tip with Disperser
Gasket - provides air tight seal
Disperser
Frit
ToT Solid Phase Extraction
- SPE for viscous sample matrices
- Customer can provide their own sorbent
- Compatible with a variety of sorbent chemistries
- Ideal for nanoparticle and antibody-conjugate drug formulation analyses
• ToT SPE Anatomy and Advantages
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• ToT SPE Method Workflow
Tip-on-Tip Solid Phase Extraction provides a fast, automated INTip solution for a variety of applications including immunoaffinity. ToT SPE is ideal for applications that require specific incubation conditions between sorbent and sample solution.
ToT SPE utilizes a bottom Single Phase Filtration tip with a disperser. The disperser provides optimal dispersive solid phase extraction conditions. This tip has two frit options available: A low retention 50 μm frit and 30 μm frit.
Products
The ToT product line is compatible with Hamilton Robotics systems. Filtration tips come in different varieties to support ToT methods for Filtration, Cleanup and SPE. DPX also offers a accessories to support adopting ToT methods.
Dual Phase Filtration Tips
Catalog Number: DPX170037
Two filters in series provide maximum filtration without clogging for higher volume blood and serum protein precipitation applications.
Compatible with all cannabis matrices (dried flower, edibles, extracts and concentrates) for pre-filtration prior to downstream analysis.
Low Porosity Filtration Tip <1 μm
Catalog Number: DPX170068
High efficiency sub-micron matrix filtration including urine, blood, and serum.
Compatible with all cannabis matrices (dried flower, edibles, extracts and concentrates) for pre-filtration prior to downstream analysis.
Catalog Number: DPX170068U
Low Porosity Filtration tips- Ultra Pure
Ultra pure grade for low detection level methods
Single Phase Filtration Tip
- This tip is compatible with Cleanup and SPE methods
- Sorbent customization available
- This tip is sold blank. Sorbent not included.
Minimal dead volume ideal for small volume blood and serum protein precipitation applications.
Single Phase Filtration Tip with a Disperser
- Disperser allows optimal solid phase extraction conditions for SPE Methods
- Sorbent customization available
- This tip is sold blank. Sorbent not included.
- Two frit options for variety of particle sizes
Sorbent Well Plate
SPS Format Plate Pre-loaded with sorbent for ToT SPE methods
Customize the sorbent mass available in a 96 deep well-plate for use with ToT SPE methods. These can contain any DPX sorbent in a range of masses per well. Contact us for sorbent options not listed.
- WAX
- WCX
- SAX
- SCX
- RP
- C18
- Silica
- Mixed Mode
- HybridSPE® – Phospholipid removal