SEC: INTip™ Size Exclusion Chromatography
Unique pipette tip design for automated gel-filtration. Convert manual desalting workflows into “walk-away” automated methods.
Our size exclusion chromatography (SEC) pipette tips are filled with a dry resin media for easy room temperature storage. Our patent-pending automated INTip Swelling method hydrates the resin to create a perfectly equilibrated gel-filtration column inside the pipette tip. No manual preparation steps are required.
Technology designed for true “walk-away” automation
- Shipped with dry resin media
- Automated resin hydration and column equilibration
- No pre-automation steps. No centrifugation. No manual column packing or slurry prep.
Advantages:
- Robust, highly reproducible methods
- Available in 1 mL and 300 µL Hamilton style formats (shown here)
- Customize gel-filtration column volume based on sample volume
- Sample volumes from 50 – 250 µL
- Save time with fast methods for protein separation
Learn more about how gel-filtration inside our pipettes tips works for protein separation in this article-
Dry resin media for SEC tips can be hydrated with water or buffer to be used for desalting and/or buffer exchange. Gravity flow or automated positive pressure forces liquid through the pipette tip column to pull the sample through the gel. As the sample passes through the column, the small molecules take the slower path through the pores of the resin while the larger protein separates from the small molecules. The protein target fraction is eluted through a dispense step. Positive pressure is used for each step of the workflow, facilitating a fast, robust automated method.
Data and Performance
BSA and Tartrazine
Absorbance values at 280 nm for protein and 425 nm for tartrazine were performed on a nanodrop one spectrophotometer. Measurements were taken for the starting material (A) after 250 µL sample application (b) and 300 µL elution (C) Results are displayed as the average % protein concentration and average absorbance value of tartrazine at 425 nm.
This short video provides an overview of size exclusion chromatography with pipette tips. The present device and methods were developed to overcome the complexities and time commitment of manual column preparation and centrifugation steps.
Resources
- Technical Note: INTip Size Exclusion Chromatography
- Technical Note: Guanidine Buffer Exchange
- Application Note: Automated Desalting and Buffer Exchange of Human IgG
- Webinar: How to use INTip Size Exclusion for desalting and buffer exchange
- Poster Presentation: Method for Screening over 375 Drugs in Blood Using INTip Size Exclusion Chromatography
- Application Note: Analysis of Comprehensive Drugs of Abuse in Oral Fluid using INTip SEC
How to Order
Choose your format, molecular weight cutoff and tip size to incorporate the amount of gel resin media within SEC tips for sample volumes up to 250 μL. Tips can be manually processed with gravity flow and have a removable cap instead of a piercable barrier.
