SEC: INTip™ Size Exclusion Chromatography
Patent-pending pipette tip design for automated group separations of complex biological mixtures. The SEC pipette tip incorporates a novel INTip swelling and packing of the gel filtration media.
Anatomy
Workflow
Our size exclusion chromatography product line can be used for desalting and buffer exchange: a group separation method where small molecules (such as salts) are separated from a group of larger molecules (such as proteins). Samples can be quickly prepared for other downstream chromatography techniques and assays. Our patent pending INTip swelling method, for the column packing, prepares the gel resin bed for sample loading in less than 10 minutes.
Piercable Barrier
Swollen resin bed ~4.3 cm in length for 200 µL sample volume
Dry resin media in the column
Technology Advantages:
- Robust highly reproducibles methods for desalting and buffer exchange
- INTip automated swelling- No manual slurry prep or column packing!
- No centrifugation or positive pressure manifolds
- Prepare large molecule analytes for downstream analysis
Anatomy
Group Separation- BSA and Tartrazine
Absorbance values at 280 nm for protein and 425 nm for tartrazine were performed on a nanodrop one spectrophotometer. Measurements were taken for the starting material (A) after 250 µL sample application (b) and 300 µL elution (C) Results are displayed as the average % protein concentration and average absorbance value of tartrazine at 425 nm.
Value of BSA Tartrazine Before and After INTip SEC
This short video provides an overview of size exclusion chromatography with pipette tips. Size exclusion chromatography pipette tips provide a solution for automating desalting and buffer exchange without the need for centrifugation or positive pressure manifolds. The present device and methods were developed to overcome the complexities and time commitment of manual column preparation and centrifugation steps.
INTip Size Exclusion Methods are currently compatible with Hamilton Robotics Systems
1 mL SEC tip
The image above shows the barrier before and after (pierced)
Available Products:
| Molecular Weight Cutoff | 1500 Da or 5000 Da |
|---|---|
| Sample Volume | 50-250 µL |
| Compatibility | Hamilton Robotics |
| Applications |
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