SEC: INTip™ Size Exclusion Chromatography
Unique pipette tip design for automated group separation of complex sample mixtures and patent-pending INTip Swelling method.
Our size exclusion chromatography (SEC) pipette tips are filled with a dry resin media for easy room temperature storage. DPX has a patent-pending method for automated INTip Swelling to hydrate the resin to create a perfectly equilibrated gel-filtration column inside the pipette tip. No centrifugation steps or manual preparation steps are required.
Technology Advantages:
- Robust, highly reproducible methods- more reproducible than manual spin columns!
- No manual slurry prep or column packing
- No centrifugation or positive pressure manifolds
- No pre-automation steps
- Room temperature storage
- Available in 1 mL and 300 µL Hamilton style formats (shown here)
- Product customization for gel-filtration column volume based on sample volume
- Sample volumes from 50 – 250 µL
- Reduced workflow times
Learn more about how gel-filtration inside our pipettes tips works for size exclusion chromatography applications in this article-
Dry resin media for SEC tips can be hydrated with water or buffer to be used for desalting and/or buffer exchange. Gravity flow or automated positive pressure forces liquid through the pipette tip column to pull the sample through the gel. As the sample passes through the column, the small molecules take the slower path through the pores of the resin while the larger protein separates from the small molecules. The protein target fraction is eluted through a dispense step. Positive pressure is used for each step of the workflow, facilitating a fast, robust automated method.
Data and Performance
BSA and Tartrazine
Absorbance values at 280 nm for protein and 425 nm for tartrazine were performed on a nanodrop one spectrophotometer. Measurements were taken for the starting material (A) after 250 µL sample application (b) and 300 µL elution (C) Results are displayed as the average % protein concentration and average absorbance value of tartrazine at 425 nm.
This short video provides an overview of size exclusion chromatography with pipette tips. The present device and methods were developed to overcome the complexities and time commitment of manual column preparation and centrifugation steps.
Resources
- Technical Note: High Throughput Group Separations
- Technical Note: Guanidine Buffer Exchange
- Application Note: Automated Desalting and Buffer Exchange of Human IgG
- Webinar: How to use INTip Size Exclusion for desalting and buffer exchange
- Poster Presentation: Method for Screening over 375 Drugs in Blood Using INTip Size Exclusion Chromatography
How to Order
Choose your format, molecular weight cutoff and tip size to incorporate the amount of gel resin media within SEC tips for sample volumes up to 250 μL. Tips can be manually processed with gravity flow and have a removable cap instead of a piercable barrier.
