INTip™ Solid Phase Extraction (SPE)

INTip SPE utilizes a patented technology known as Dispersive Pipette XTRaction. This device is unique from all other SPE devices because sorbent is loosely contained within a pipette tip.

The disperser helps to perturb the sample solution and loose sorbent during aspirate and dispense steps. This mixing provides a highly efficient interaction of the sorbent and sample solution resulting in ideal analyte recoveries and reduced matrix effects. You can seamlessly integrate XTR tips with any workflow or method.

INTip SPE

XTR - Universal style 1 mL tip

Barrier

Disperser

Loose Sorbent

Frit

• XTR Tip Anatomy

• Cleanup Workflow

• Bind-Wash-Elute Workflow

• Aspirate/Dispense Step

Click links to sort the slider!

Dispersive
XTRaction
SPE
Cartridge
96-well
plate
Customization
High efficiency
Low sample
volumes
Reduced solvent
volumes
Scalable
Process 1 - 96
Automation
No hardware
required

Aspirate Sample

Easy Sample Cleanup

Mix sample via aspirate and dispense steps

Interferents Bind to Sorbent

Dispense Analyte
of Interest

  • No vacuum manifold
  • Unparalleled ease of use
  • Low cost, automated solution
  • Custom method development

Bind - Wash - Elute

Condition

Bind

Disperser forces turbulent mixing of loose sorbent

Interferents Wash Off

Elute Analyte of Interest

Analyte Binds
to Sorbent

Wash

Elute

  • Rapid extraction times
  • Higher analyte recoveries
  • Sorbent chemistry customization
XTR μElution -300 μL size tip

Product Description

μXTR Tips utilize a proprietary design to provide fast dispersive solid-phase XTRactions with minimal elution volumes for maximum concentration effects. Micro-elution tips are available with 1-3 mg of sorbent and utilize a unique low-retention frit. This allows for elution volumes as low as 25 μL, thereby eliminating time-consuming solvent evaporation steps. Currently this tip is available in Hamilton, or Integra formats. 

Advantages:

  • Same advantages as XTR
  • Eliminate solvent evaporation/dry down step
  • Increase sensitivity
  • Reduce elution volumes to as low as 25 μL for up to 8X concentration factor
  • No positive pressure/vacuum manifold
  • Compatible with all sorbents with > 50 μm particle size.
sample preparation for urine for mass spectrometry analysis

Urine

sample preparation for blood for mass spectrometry analysis

Blood/Serum

sample preparation for oral fluid for mass spectrometry analysis

Oral Fluid

Tissue

sample preparation for meconium for mass spectrometry analysis

Meconium

Sample preparation for hair samples for mass spectrometry analysis

Hair

Food Samples

Plant Material

Sort through
applications by
matrix type!

Method/Matrix Flexibility

XTR and μXTR tips can be used for cleanup or bind-wash-elute protocols for a wide variety of sample preparation applications. The applications page on our website has details for applications using our tips by industry. We also have a Product Guide by Matrix that can help you determine the use of DPX products.

If you don’t see a specific application listed then please contact us for custom method development! Pipette tips are easy to use and can be integrated with your existing workflow

Dispersive Pipette XTRaction supports:

  • Better quality data
  • Fewer repeat batches
  • Scale production
  • Low sample volumes
  • Less internal standard, solvent, enzyme, evaporation time & instrument maintenance

XTR - Universal style 1 mL tip

Barrier

Disperser

Loose Sorbent

Frit

Aspirate Sample

Mix Sample

Interferents Bind to Sorbent

Dispense Analyte
of Interest

Product Description

This technology enables INTip™ chemistry for easy sample preparation by incorporating solid phase extraction within a pipette tip. The disperser helps to perturbate the sample solution and sorbent during aspirate and dispense steps. This mixing provides a highly efficient interaction of the sorbent with the analyte of interest resulting in ideal analyte recoveries.

You can seamlessly integrate dispersive pipette XTRaction into any workflow or method. Pipette tips are available in manual, semi-automated or fully automated formats.

Sorbent chemistry is completely customizable. Depending on the format XTR tips can hold 1 – 100 mg of sorbent.

Advantages:

  • Easy Sample Cleanup
  • No vacuum manifold
  • Custom method development
  • Low cost automated solution
  • Rapid extraction times
  • Sorbent chemistry customization
  • Higher analyte recoveries
XTR μElution -300 μL size tip

Product Description

μXTR Tips utilize a proprietary design to provide fast dispersive solid-phase XTRactions with minimal elution volumes for maximum concentration effects. μXTR tips are available with 1-3 mg of sorbent and utilize a unique low-retention frit. This allows for elution volumes as low as 25 μL, thereby eliminating time-consuming solvent evaporation steps. Currently this tip is available in Hamilton, or Integra formats. 

Advantages:

  • Same advantages as XTR
  • Eliminate solvent evaporation/dry down step
  • Increase sensitivity
  • Reduce elution volumes to as low as 25 μL for up to 8X concentration factor
  • No positive pressure/vacuum manifold
  • Compatible with all sorbents with > 50 μm particle size.
sample preparation for urine for mass spectrometry analysis

Urine

sample preparation for blood for mass spectrometry analysis

Blood/Serum

sample preparation for oral fluid for mass spectrometry analysis

Oral Fluid

Tissue

sample preparation for meconium for mass spectrometry analysis

Meconium

Sample preparation for hair samples for mass spectrometry analysis

Hair

sample preparation for food safety applications

Food Samples

cannabis material sample preparation

Plant Material

Sort through
applications by
matrix type!

Method/ Matrix Flexibility with INTip Sample Preparation

XTR and μElution XTR tips can be used for cleanup or solid phase extraction protocols for a wide variety of sample preparation applications. The applications page on our website has details for applications using our tips by industry: Clinical, Forensic, Food Safety, Proteomics and Genomics. We also have a product guide by matrix.

If you don’t see a specific application listed then please contact us for custom method development! Pipette tips are easy to use and can be integrated with your existing workflow

Dispersive Pipette XTRaction supports:

  • Better quality data
  • Fewer repeat batches
  • Scale production
  • Low sample volumes
  • Less internal standard, solvent, enzyme, evaporation time & instrument maintenance

Download Product
Ordering Guide

INTip SPE vs. Traditional SPE

Videos

High Quality Sorbents

DPX offers sorbents chemistries in a range of phases to capture unique selectivity for a diverse spectrum of analytes. XTR tips are manufactured to order per custom specifications. They can hold 1 – 100 mg of sorbent depending on the tip format.

Our reversed phase sorbent is a styrene divinylbenzene polymer with an average particle size of 75 μm. It is ideal for nonpolar and slightly polar compounds.

Download Application Notes:

Our weak anion exchange (WAX) sorbent has a secondary amine phase with a styrene divinyl benzene backbone for the binding of strong acids, such as sulphates. WAX sorbents are offered in 10-20 μm and 55-65 μm particle size ranges.

Download Application Notes:

Our weak cation exchange (WCX) sorbent has a carboxylic acid stationary phase with a styrene divinyl benzene backbone for the binding of strong bases such as quaternary amines. WCX sorbent is offered in a 30-70 μm particle size range.

Our strong cation exchange (SCX) sorbent has a sulfonated stationary phase with a styrene divinyl benzene backbone for the binding of basic compounds. SCX sorbents are offered in 10-20 μm, 25-35 μm and 55-65 μm particle size ranges.

Download Application Notes:

Our strong anion exchange (SAX) sorbent has a quaternary amine phase with a styrene divinyl benzene backbone for the binding of acidic compounds. SAX sorbent is offered in a 55-65 μm particle size range.

Our silica sorbent is amenable to extraction via hydrogen bonding. It is available in a 40-60 μm particle size range.

Our C18 sorbent has a silica gel backbone. It is endcapped, with a carbon load of 18-22%, and 40-60 μm particle size range.

DPX is proud to offer HybridSPE® (a Supelco/Sigma product) sorbent. It is a proprietary zirconia coated silica that selectively removes phospholipids from biological samples.

  • Unique chemistry for excellent recovery of both hydrophobic and hydrophilic analytes

Download Application Notes:

Our mixed mode products are completely customized by choosing any of the above chemistries to combine in one tip. Each sorbent’s mass can also be customized for optimal extraction characteristics for any analyte/matrix combination.

Ex. WAX/RP, WAX/SCX

Download Application Notes:

Combine Dispersive Pipette XTRaction and SALLE (Salting-out Assisted Liquid-Liquid Extraction) for a novel cleanup mechanism. XTR tips can house sorbents like WAX or RP for cleanup and the salt (S) necessary for SALLE. This methodology is ideal for applications that require acetonitrile for protein precipitation and are focused on hydrophobic analytes.

Ex. WAX-S, RP-S

Dispersive Pipette XTRaction + SALLE

Aspirate and dispense steps mix sample solution and loose sorbent material. Added salt (S) assists in phase separation during extraction.

Download Application Notes:

Learn more about WAX-S Methods

Tip Formats

The XTR product line is available in a variety of formats to be used with manual, semi-automated or fully automated liquid handlers.

Compatibility:
  • Microlab NIMBUS, STAR, and VANTAGE systems – all sizes and configurations
Pipette Tip Sizes:
  • 300 μL
  • 1 mL
  • 300 μL µXTR – (micro-elution tip)

XTR tips for Hamilton utilize the CO-RE® Technology. Custom method development is available.

Compatibility:
  • Evolve, Voyager, VIAFLO ASSIST, and VIAFLO96 systems
Pipette Tip Sizes:
  • 300 μL
  • 1250 μL
  • 300 μL µXTR – (micro-elution tip)
XTR - 1 mL style GERSTEL tip

If you have a Gerstel MPS system and want to try XTR tips please contact Gerstel.

Pipette Tip Size:
  • 1 mL
XTR - 1 mL style epT.I.P.S

epT.I.P.S.® – for epMotion

  • 1 mL
XTR - Universal style 1 mL tip

Universal – Manual

This tip fits most single or multi-channel pipettors.

Pipette Tip Sizes:
  • 1 mL – Fits single and multichannel pipettes and the DPX 1 mL pneumatic extractor.
  • 5 mL – Comes with a syringe for manual use. This is the largest tip offered.

Pneumatic Extractor

DPX offers a 1 mL Pneumatic Extractor. This devices drives syringes to create positive and negative pressure to aspirate and dispense solutions in and out of universal style tips. The Pneumatic Extractor can process 24 – 96 samples at one time.

If you know exactly what options you want from these choices then feel free to fill out the product ordering form below and specify all the options.

Interested in XTR Tips?

If you are unsure which XTR Tip and sorbent to request for your protocol, please consider filling out a product request form. Our team of application scientists will be able to better recommend a product or help you with custom method development with your sample matrix, volume and method parameters provided. We offer confidential method development and on-site technical support to help you get through the validation process.

  • Sorbent available in custom amounts. Please let us know which sorbent and how much you want in your tip.