Extraction and purification of genomic DNA (gDNA) is a critical step in molecular biology and genomics workflows. The quality, purity, and yield of isolated gDNA directly affect downstream applications such as sequencing, mutation screening, and genetic engineering. While manual nucleic acid extraction methods remain common due to their accessibility and low cost, they are often labor-intensive, time-consuming, and prone to contamination or variability. Increasing demands for throughput, consistency, and automation have driven the development of more efficient solutions.

DPX Technologies has developed NiXTips (patent-pending) to address these needs. Designed for use on automated liquid handlers, NiXTips enable fully automated isolation of high-quality gDNA from diverse sample types, including adherent and suspension cell lines. The technology uses a proprietary microporous media that integrates lysis, binding, washing, and elution within a single pipette tip. This enclosed format minimizes contamination risk, simplifies workflow design, and streamlines high-throughput processing. In this study, we demonstrated the performance of 300 μL Hamilton NiXTips for automated gDNA extraction. Results highlight the ability of the system to deliver reproducible yields of pure gDNA with minimal user intervention. Collectively, these features position NiXTips as an efficient, scalable alternative to manual extraction methods, enabling high-quality gDNA isolation for demanding genomic applications.

For complete results using this method you can download the application note.

NiX tips provide automated genomic sample preparation for Next Generation Sequencing library preparation methods and other downstream analysis. Our innovative NiX tips utilize a proprietary microporous media and buffer system to facilitate Solid Phase Reversible Immobilization (SPRI) methods and allow for fast and effective removal of primers, primer, dimers, dNTPs, unincorporated labeled nucleotides, enzymes, and salts from PCR and other reaction mixtures.

Learn more about NiX tips used in this method.