At present, automated next-generation sequencing (NGS) library preparation workflows predominantly rely on super-paramagnetic beads and plate-based magnets for sample cleanup and fragment size-selection. A typical bead-based cleanup method requires six pipette tips per sample, a labware transport mechanism, and fine-tuning of pipetting heights to ensure complete supernatant removal from pelleted beads. DPX Technologies’ NiXTips contain immobilized silica media within robotic pipette tips and offer an automated bead-free alternative for cleanup and size-selection. The NiXTip workflow requires only two pipette tips per sample and five fewer deck positions than bead-based methods. In this study, we compared the performance of NiXTips against beads in two NGS library preparation workflows, including downstream sequencing analysis. In all areas, NiXTips had equivalent or superior performance compared to beads, demonstrating the suitability of using NiXTips for NGS library preparation workflows.

For complete results using this method you can download the application note.

NiX tips provide automated genomic sample preparation for Next Generation Sequencing library preparation methods and other downstream analysis. Our innovative NiX tips utilize a proprietary microporous media and buffer system to facilitate Solid Phase Reversible Immobilization (SPRI) methods and allow for fast and effective removal of primers, primer, dimers, dNTPs, unincorporated labeled nucleotides, enzymes, and salts from PCR and other reaction mixtures.

Learn more about NiX tips used in this method.