• DNA fragment size selection is a critical step in sequencing library preparation, directly influencing read quality and downstream performance.
• Conventional bead-based double-sided methods can introduce variability through multiple transfers and increased handling.
• 300 μL NiXTips streamline this process by performing binding, washing, and elution entirely within the pipette tip, minimizing loss and cross-contamination.
• On the Hamilton STARlet, the automated double-sided workflow enables consistent enrichment of DNA fragments within the desired range.

For complete results using this method you can download the application note.

NiX tips provide automated genomic sample preparation for Next Generation Sequencing library preparation methods and other downstream analysis. Our innovative NiX tips utilize a proprietary microporous media and buffer system to facilitate Solid Phase Reversible Immobilization (SPRI) methods and allow for fast and effective removal of primers, primer, dimers, dNTPs, unincorporated labeled nucleotides, enzymes, and salts from PCR and other reaction mixtures.

Learn more about NiX tips used in this method.