SLAS 2024 Poster Authors: Julia Portocarrero¹, John Russel¹, Michael Gonzalez¹, Matt Fits², Paul Meeh², William E. Brewer²

1. Life Edit Therapeutics 2. DPX Technologies 

Automation Platform: Agilent Bravo

This method demonstrates NiX tips for PCR cleanup produces comparable results to bead-based cleanup in both amount of recovered DNA product and speed of the cleanup protocol. However, NiX tips are superior for high throughput walk-away solutions as they are shelf stable and require no extra handling considerations nor hardware requirements. Magnetic beads are the gold standard for PCR cleanup in NGS processes. However, they require specific handling that can make high throughput automation difficult.

PCR product was made using primers targeting the β2M locus and HEK cell whole-genome DNA template. PCR reactions were amplified for 35 cycles, pooled, and analyzed on a TapeStation prior to input into cleanups. For NiX tip cleanups, 25 μl of PCR product was mixed with DPX binding buffer at a 1x ratio, and mixed for variable binding cycles with NiX tips (NiX Tips, DPX Technologies, Columbia SC). NiX tips were then washed twice in 80% ethanol, and eluted with 25 μL of water.

For complete results using this method you can download the application note.

NiX tips provide automated genomic sample preparation for Next Generation Sequencing library preparation methods and other downstream analysis. Our innovative NiX tips utilize a proprietary microporous media and buffer system to facilitate Solid Phase Reversible Immobilization (SPRI) methods and allow for fast and effective removal of primers, primer, dimers, dNTPs, unincorporated labeled nucleotides, enzymes, and salts from PCR and other reaction mixtures.

Learn more about NiX tips used in this method.