Saliva collection for genomic DNA extraction is a convenient and non-invasive procedure that is commonly used for genomic studies. However, saliva is a heterogeneous biofluid containing mucins, enzymes, microbes and inhibitory polysaccharides that presents inherent challenges for nucleic acid purification. Saliva collection tubes containing nucleic acid stabilization buffers have allowed for self collection and storage at room temperature. For high throughput laboratories processing hundreds to thousands of saliva samples, an automated process for nucleic acid extraction using a liquid handler is required.

Commercial nucleic acid extraction protocols that employ protein precipitation as a cleanup prior to nucleic acid purification are not amenable to automation. Bind-wash-elute protocols utilizing magnetic beads have been developed but require additional hardware for processing. As an alternative, DPX Technologies has developed an automated DNA extraction protocol utilizing Oragene collection kits and patent-pending NiXTips on a Hamilton® liquid handler. Integration of all steps within the pipette tip reduces handling complexity, improves reproducibility, and ensures recovery of DNA with sufficient integrity for downstream molecular analysis. The demonstrated combination of yield, integrity, and functional performance establishes this method as a robust alternative to conventional workflows and a valuable tool for laboratories engaged in high-throughput genomic research.

For complete results using this method you can download the application note.

NiX tips provide automated genomic sample preparation for Next Generation Sequencing library preparation methods and other downstream analysis. Our innovative NiX tips utilize a proprietary microporous media and buffer system to facilitate Solid Phase Reversible Immobilization (SPRI) methods and allow for fast and effective removal of primers, primer, dimers, dNTPs, unincorporated labeled nucleotides, enzymes, and salts from PCR and other reaction mixtures.

Learn more about NiX tips used in this method.