High-quality genomic DNA (gDNA) is critical for downstream molecular biology and genomic applications. While magnetic bead technology has been widely adopted for gDNA extractions, bead capture time, bead carryover, bead resuspension and requirements for magnets complicate the process. Increasing demands for consistency and automation have driven the development of more streamlined extraction solutions. DPX Technologies’ patent-pending NiXTips® address these challenges. Designed for automated liquid handlers, NiXTips enable fully automated isolation of high-quality gDNA from whole blood. The proprietary microporous media integrates bind, wash, and elute steps within a single pipette tip, reducing consumable use, minimizing contamination risk, and supporting high-throughput processing. We evaluated 1 mL Hamilton NiXTips for automated gDNA isolation from human whole blood. Results demonstrate reproducible recovery of pure, high-molecular-weight gDNA across variable blood inputs with minimal user intervention, highlighting NiXTips as a scalable alternative to bead-based methods.

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NiX tips provide automated genomic sample preparation for Next Generation Sequencing library preparation methods and other downstream analysis. Our innovative NiX tips utilize a proprietary microporous media and buffer system to facilitate Solid Phase Reversible Immobilization (SPRI) methods and allow for fast and effective removal of primers, primer, dimers, dNTPs, unincorporated labeled nucleotides, enzymes, and salts from PCR and other reaction mixtures.

Learn more about NiX tips used in this method.