NiXTips™: INTip Nucleic Acid Cleanup
Automated genomic sample preparation for Next Generation Sequencing library preparation methods and other downstream analysis.
Innovative Technology for Genomic Sample Preparation
Solid Phase Reversible Immobilization (SPRI) is commonly used for purifying and size selecting nucleic acids in Next Generation Sequencing (NGS) library preparation methods and a variety of applications for genomics research. Our innovative NiX tips utilize a proprietary microporous media and buffer system to facilitate SPRI methods and allow for fast and effective removal of primers, primer, dimers, dNTPs, unincorporated labeled nucleotides, enzymes, and salts from PCR and other reaction mixtures.
Boost efficiency and reproducibility with complete automation using NiX tips. Our methods requires less hands-on processing than traditional column or bead-based methods, reducing opportunities for sample contamination and experimental errors.
Process up to 384 samples in less than 30 minutes!
- Save thousands and eliminate need for ancillary equipment like centrifuges, vortex mixers, vacuum manifolds or magnet setups
- Reduce risk of nucleic acid shearing
- Pure sample ready for NGS and other downstream analysis
| NiX Tips | Magnetic Beads | Spin Column | |
|---|---|---|---|
| High Efficiency | ✅ | ✖️ | ✖️ |
| Low Cost | ✅ | ✖️ | ✅ |
| Scalable | ✅ | ✅ | ✖️ |
| Automation Compatible | ✅ | ✅ | ✖️ |
| No Hardware | ✅ | ✖️ | ✖️ |
Full method video coming soon! This is a short clip of NiX tips on a Hamilton Nimbus system moving over to the bind step of the method.
Size Selection and PCR Cleanup Data using NiX Tips
Average Yield Based on Number of Binding Cycles
The chart above shows close to 100% yield with 40 or more binding cycles based on the response of 4 fragments ranging from 325-510 bp. Applications requiring less yield can reduce binding cycles for even faster workflows.
Sanger sequencing of replicates of an amplicon from the human GAPDH gene serves as a validation, affirming the efficacy and quality of NiX tips for PCR cleanup.
DNA Yield
Comparison of DNA yield using different PCR master mixtures.
Left Side Size Selection
The ratio of binding buffer to sample solution can provide optimization of specific removal of desired range of bp. In the chart on the left, a 25 μL sample was utilized with a DNA MWM ranging from 19 bp-1164 bp. The amount of binding buffer was decreased by the fraction shown to reduce recovery of the smaller DNA fragments.
NiX Methods are currently compatible with Hamilton Robotics Systems and Agilent Bravo. Kit contents include tips and binding buffer.
Specifications:
| Downstream applications | PCR reactions and other DNA enzymatic reactions |
|---|---|
| Starting Material | dsDNA, DNA fragments, PCR products |
| Starting Amount | 20-25 µL |
| Elution Volume | 25-50 µL |
| Separation Range | > 100 bp |
| DNA Recovered | up to 100% depending on fragment size and buffer composition |
| Binding Capacity | 40 µg |
| Processing Mode | Manual, automated |
| Throughput | 1- 384 depending on tip format |
| DNA Binding Technology | Proprietary silica media |
| Processing Time | 25 min. |
| Special Notes | Different PCR master mixtures affect binding and yield of DNA fragments |