Genomic Sample Preparation

Automated INTip methods for nucleic acid purification from complex biological matrices and cleanup for downstream analysis

Technology Advantages

MPX technology for genomics sample preparation utilizes proprietary silica media within functionalized pipette tips (NiXTips) to facilitate methods for DNA extraction from biological sample and methods for PCR cleanup or size selection.

Proprietary Silica Media

	NiXTips	Magnetic Beads	Spin Column
High Efficiency	✅	✖️	✖️
Cost Effective	✅	✖️	✅
Scalable	✅	✅	✖️
Automation Compatible	✅	✅	✖️
No Hardware	✅	✖️	✖️

INTip Nucleic Acid Purification and Cleanup methods are powered by MicroPorous Xtraction (MPX) Technology

Hamilton style tip shown here

Extraction Workflow

Pre-treatment

Wash 2

Elute

Wash 1

Bind

Add IPA

DNA binds to silica media

Extraction Workflow

Extraction Performance

High capacity media allows for concentration factors comparable to traditional spin column or magnetic bead workflows with a simple bind-wash-elute protocol.

Process up to 96 samples from lysate to purified DNA in as little as 20 minutes

High yield up to 43 µg total genomic DNA

Methods for extraction from whole blood, cell cultures and saliva

Direct comparison of average DNA yields from 250 μL of blood with DPX method for isolation using 1 mL NiXTips versus the leading bead-based and column-based.

Example data for a 250 µL sample volume using a 1 mL NIXTip. NiX methods can support 50-500 µL sample volume.

Comparison of gDNA yields (ng/μL or total ng) from 300 μL NiXTips vs. bead-based method across the 5 dilutions, based on qPCR data from QuantStudio 3.

Cleanup Workflow

Solid Phase Reversible Immobilization (SPRI) is commonly used for purifying and size selecting nucleic acids in Next Generation Sequencing (NGS) library preparation methods and a variety of applications for genomics research. NiXTips utilize a proprietary microporous media and buffer system to facilitate SPRI methods and allow for fast and effective removal of primers, primer-dimers, dNTPs, unincorporated labeled nucleotides, enzymes, and salts from PCR and other reaction mixtures.

Application Notes:

Cleanup Workflow

PCR Cleanup and Size Selection Performance

Recovery of DNA MWM VIII in ng/μL with different (5, 10, 15, 20, 25, 30) aspirate/dispense cycles (binding cycles).

Percent recovery of DNA fragments at different binding buffer to sample ratios.

Product Guide

Optimizing DNA Extraction

Videos

How to Order

NiXTips are compatible with major liquid handling robotic platforms. NiXTips are sold in matrix-specific kits for DNA extraction and prep kits for cleanup methods, including necessary buffers. Download product specifications by clicking the applications available. Contact us for custom method development for applications not listed.