Project Description

A Sensitive Liquid Chromatography–Tandem Mass Spectrometry Method for the Measurement of 11β-MNT and 11β-MNTDC in Human Serum

-Summary of MSACL 2017 Poster Presentation by: Feng Bai, Vignesh Iyer, Ronald Swerdloff, and Christina Wang; Los Angeles Biomedical Research Institute at Harbor-UCLA Medical Center

HybridSPE® Extraction

11-beta-methyl-19-nortestosterone (11β-MNT) is the active androgen derived from 11-beta-methyl nortestosterone dodecylcarbonate (11β-MNTDC). 11β-MNT is being developed as a candidate drug of male hormonal contraception. 11β-MNT like dimethandronlone (DMA) binds to both the androgen and progesterone receptor and may provide the suppression of spermatogenesis with one compound instead of two agents, testosterone and a progestin. The objective of this study was to develop and validate a highly sensitive LC-MS/MS method for the simultaneous detection of serum 11β-MNT and 11β-MNTDC levels for research and clinical purposes in men.

Protein Precipitation Serum Sample + I.S. + ACN
Vortex & Centrifuge (for increased efficiency use Tip-on-Tip)
Transfer supernatant
Condition

Aspirate and Dispense HybridSPE- XTR Tips with 500 μL acetonitrile

Extraction

Aspirate and dispense the supernatant 2x with HybridSPE- XTR Tips to bind interferents to sorbent

Transfer remaining clean sample to a new glass vial

Prep for injection

Dry down the extracted samples

Reconstitute with 40% MeOH in 0.1% formic acid H2O for the analysis

You can automate this method with Tip-on-Tip for protein precipitation and HybridSPE XTR tips.

sample preparation for blood/ serum samples prior to LC-MS/MS analysis

INTip Solutions

Combines a top wide bore conductive tip and a bottom tip for filtration. Filtration tips are available on Hamilton and Integra platforms. Tip-on-Tip Technology was designed to automate and simplify complex sample preparation.

XTR tips can contain loose HybridSPE sorbent material in 30 mg or 50 mg depending on the tip format. Tips are available for Tecan, Hamilton, Integra and Universal style for multi-channel pipettors.

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