Quantitation of Fentanyl, Norfentanyl, Buprenorphine, and Norbuprenorphine in Urine using WAX-XTR tips and LC-MS/MS
Extraction < 15 minutes
Automation Compatibility: Hamilton
Fentanyl and buprenorphine are potent analgesics making them medicinally appealing and easy to abuse. Thus, they are two of the most common analytes seen in clinical and forensic laboratories. Due to their high potency and low dosages, urinary concentrations are much lower than other common opiates/opioids. This leaves most laboratories struggling to increase the sensitivity and speed of their methods. This method using INTip™ SPE (powered by Dispersive Pipette XTRaction technology) with weak anion exchange (WAX) sorbent promotes an easy, fast, and sensitive method to help laboratories increase efficiency of detecting these compounds and their metabolites.
Sample Preparation Workflow
|CONDITION||Aspirate/ dispense 30% MeOH|
|BIND SAMPLE||Aspirate/ dispense x5 with WAX – XTR tips|
|WASH||Aspirate/ dispense 400 µL water from 2 aliquots|
|ELUTE||Aspirate / dispense ACN x3|
To order the exact product in this application use this catalog number.
Catalog: DPX170117 Description: XTR tips: 10 mg WAX (60µm) in 1 mL Hamilton format
To customize this product choose sorbent amount and tip format from available options.