SEC: INTip™ Size Exclusion Chromatography
Unique pipette tip design for automated group separation of complex sample mixtures and patent-pending INTip Swelling method.
Our size exclusion chromatography (SEC) pipette tips can be used for desalting and buffer exchange: a group separation method where small molecules (such as salts) are separated from a group of larger molecules (such as proteins). Samples can be quickly prepared for other downstream chromatography techniques and assays. SEC tips are available in 1 mL and 300 µL Hamilton style formats (shown here).
SEC tips facilitate our patent pending INTip swelling method for the column packing which prepares the gel resin bed for sample loading in less than 2 minutes.
Technology Advantages:
- Robust highly reproducible methods
- No manual slurry prep or column packing
- No centrifugation or positive pressure manifolds
- No pre-automation steps
- Room temperature storage
Data and Performance
BSA and Tartrazine
Absorbance values at 280 nm for protein and 425 nm for tartrazine were performed on a nanodrop one spectrophotometer. Measurements were taken for the starting material (A) after 250 µL sample application (b) and 300 µL elution (C) Results are displayed as the average % protein concentration and average absorbance value of tartrazine at 425 nm.
This short video provides an overview of size exclusion chromatography with pipette tips. The present device and methods were developed to overcome the complexities and time commitment of manual column preparation and centrifugation steps.
Resources
- Technical Note: High Throughput Group Separations
- Technical Note: Guanidine Buffer Exchange
How to Order
Choose your format, molecular weight cutoff and tip size to incorporate the amount of gel resin media within SEC tips for sample volumes up to 250 μL. Tips can be manually processed with gravity flow and have a removable cap instead of a piercable barrier.
