Simplified Solid Phase Extraction
Improve analyte recoveries and increase efficiency
with seamless automation– all in a simple pipette tip format
Improve sample preparation for LC-MS/MS analysis with INTip SPE for cleanup and extraction
Unlike traditional SPE, XTR tips utilize a patented Dispersive Pipette XTRaction technology. Sorbent chemistry is loosely contained within pipette tips; during aspirate and dispense cycles, the disperser actively mixes sample and sorbent, increasing interaction efficiency.
The result:
- Increased recoveries
- Improved reproducibility
- Eliminate channeling effects
- Eliminate hardware
- Fast methods with simplified workflows
Cleanup and Solid Phase Extraction with XTR tips
XTR tips provide a fast, automated solution to streamline sample preparation for urine, blood, tissue, oral fluid, meconium, hair, food samples and plant material.
Solid Phase Extraction Workflow:
Cleanup Workflow:
Product Ordering Guide: Download
Application and Technical Notes:
- Automated Extraction of Therapeutic Drugs of Abuse in Urine
- Automated Extraction of Catecholamines from Urine
- Automated Method for Quantitation of Fentanyl, Norfentanyl, Buprenorphine and Norbuprenorphine in Urine
- Quantification of 11 cannabinoids and metabolites in urine
- Sample Preparation for Analysis of Cortisol in Urine and Saliva
- Automated SPE for Drugs of Abuse Testing in Oral Fluid
- THC Analysis in Whole Blood
- A Sensitive LC-MS/MS Method for Analysis of Male Drug Hormones in Serum
- Analysis of Benzodiazepines in 5 Biological Matrices
- Analysis of Benzodiazepines in Meconium
- JAT Article: Retrospective Analysis of Selected Opioids in Hair
- JAT Article: A Rapid Assay for Simultaneous Determination of Nicotine, Cocaine and Metabolites in Meconium
- Analysis of Potency in Cannabis Material
Product Description
This technology enables INTip™ chemistry for easy sample preparation by incorporating solid phase extraction within a pipette tip. The disperser helps to perturbate the sample solution and sorbent during aspirate and dispense steps. This mixing provides a highly efficient interaction of the sorbent with the analyte of interest resulting in ideal analyte recoveries.
You can seamlessly integrate dispersive pipette XTRaction into any workflow or method. Pipette tips are available in manual, semi-automated or fully automated formats.
Sorbent chemistry is completely customizable. Depending on the format XTR tips can hold 1 – 100 mg of sorbent.
Advantages:
- Easy Sample Cleanup
- No vacuum manifold
- Custom method development
- Low cost automated solution
- Rapid extraction times
- Sorbent chemistry customization
- Higher analyte recoveries
Product Description
μXTR Tips utilize a proprietary design to provide fast dispersive solid-phase XTRactions with minimal elution volumes for maximum concentration effects. μXTR tips are available with 1-3 mg of sorbent and utilize a unique low-retention frit. This allows for elution volumes as low as 25 μL, thereby eliminating time-consuming solvent evaporation steps. Currently this tip is available in Hamilton or Integra formats.
Advantages:
- Same advantages as XTR
- Eliminate solvent evaporation/dry down step
- Increase sensitivity
- Reduce elution volumes to as low as 25 μL for up to 8X concentration factor
- No positive pressure/vacuum manifold
- Compatible with all sorbents with > 50 μm particle size.
Method/ Matrix Flexibility with INTip Sample Preparation
XTR and μElution XTR tips can be used for cleanup or solid phase extraction protocols for a wide variety of sample preparation applications. The applications page on our website has details for applications using our tips by industry: Clinical, Forensic, Food Safety, Proteomics and Genomics. We also have a product guide by matrix.
If you don’t see a specific application listed then please contact us for custom method development! Pipette tips are easy to use and can be integrated with your existing workflow
Dispersive Pipette XTRaction supports:
- Better quality data
- Fewer repeat batches
- Scale production
- Low sample volumes
- Less internal standard, solvent, enzyme, evaporation time & instrument maintenance
Why scientists choose INTip SPE:
How to order XTR tips
XTR tips are manufactured to order per custom specifications. Choose your tip format, size, chemistry, and amount.
High-quality sorbents are available in a range of phases to capture unique selectivity for a diverse spectrum of analytes. XTR tips can hold 1 – 100 mg of sorbent depending on the format. Review chemistry options and tip formats below.
- Hamilton: 1 ml and 300 µL
- Gerstel: 1 mL
- PAL System: 1 mL
- Rainin: 1 ml and 250 µL
- Integra: 1250 µL and 300 µL
- eppendorf: 1 mL epMotion
- opentrons: 1mL
- Perkin Elmer: 1 mL
- Tecan: 1 mL and 200 µL
- Accuris, AutoMate: 1 mL
- Beckman Coulter: coming soon
- Universal/ Manual Pipette tips: 1 mL tips fit most single and multichannel pipettors
XTR tips can hold 1 – 100 mg of sorbent depending on tip size.
Reverse Phase: Our reversed phase sorbent is a styrene divinylbenzene polymer with an average particle size of 75 μm. It is ideal for nonpolar and slightly polar compounds.
Weak Anion Exchange: Our weak anion exchange (WAX) sorbent has a secondary amine phase with a styrene divinyl benzene backbone for the binding of strong acids, such as sulphates. WAX sorbents are offered in 10-20 μm and 55-65 μm particle size ranges.
Weak Cation Exchange: Our weak cation exchange (WCX) sorbent has a carboxylic acid stationary phase with a styrene divinyl benzene backbone for the binding of strong bases such as quaternary amines. WCX sorbent is offered in a 30-70 μm particle size range.
Strong Cation Exchange: Our strong cation exchange (SCX) sorbent has a sulfonated stationary phase with a styrene divinyl benzene backbone for the binding of basic compounds. SCX sorbents are offered in 10-20 μm, 25-35 μm and 55-65 μm particle size ranges.
Strong Anion Exchange: Our strong anion exchange (SAX) sorbent has a quaternary amine phase with a styrene divinyl benzene backbone for the binding of acidic compounds. SAX sorbent is offered in a 55-65 μm particle size range.
Silica: Our silica sorbent is amenable to extraction via hydrogen bonding. It is available in a 40-60 μm particle size range.
C18: Our C18 sorbent has a silica gel backbone. It is endcapped, with a carbon load of 18-22%, and 40-60 μm particle size range.
Our mixed mode products are completely customized by choosing any of the above chemistries to combine in one tip. Each sorbent’s mass can also be customized for optimal extraction characteristics for any analyte/matrix combination.
Ex. WAX/RP, WAX/SCX
Combine Dispersive Pipette XTRaction and SALLE (Salt Assisted Liquid-Liquid Extraction) for a novel cleanup mechanism. XTR tips can house sorbents like WAX or RP for cleanup and the salt (S) necessary for SALLE. This methodology is ideal for applications that require acetonitrile for protein precipitation and are focused on hydrophobic analytes.
Aspirate and dispense steps mix sample solution and loose sorbent material. Added salt (S) assists in phase separation during extraction.
HybridSPE® – A proprietary zirconia-coated silica that selectively removes phospholipids from biological samples. A unique chemistry for excellent recovery of both hydrophobic and hydrophilic analytes.
Supel™ Swift HLB: A proprietary and patent-pending copolymer having both hydrophilic and lipophilic functional groups. The sorbent’s dual polarity nature makes it ideal for extracting a broad range of compounds from aqueous matrices, and is an appropriate choice in applications for food & environmental samples as well as biological samples such as urine, serum and plasma. The hydrophilic and lipophilic balance (HLB) property of the polymer material enables the retention of a wide range of compounds having a wide range of polarities and Log P values.
How to choose the right SPE format for your application
There’s quite a few options for sample preparation products for solid phase extraction protocols. How do you choose between catridges, 96 well-plates, microelution formats and dispersive pipette extraction?
Selecting the most appropriate SPE format can have a significant impact on analytical performance, laboratory productivity and overall workflow efficiency.
Read the evaluation guide from Biotage.
