Quantitation of Synthetic Opioids In Urine

Quantitation of Fentanyl, Norfentanyl, Buprenorphine, and Norbuprenorphine in Urine using XTR Tips and LC-MS/MS

Extraction 96 samples in < 15 minutes

This method provides a fast LC-MS/MS analysis of commonly abused synthetic opioids in urine.

Fentanyl and buprenorphine are potent analgesics making them medicinally appealing and easy to abuse. Thus, they are two of the most common analytes monitored in clinical and forensic laboratories. Due to their high potency and low dosages, urinary concentrations are much lower than other common opiates/opioids. This leaves most laboratories struggling to increase the sensitivity and speed of their methods. This method uses INTip Solid Phase Extraction. XTR tips with strong cation exchange (SCX) sorbent provide an easy, fast, and sensitive extraction method to help laboratories increase efficiency of detecting these compounds and their metabolites.

Sample Preparation Workflow

CONDITION	Aspirate/ dispense 30% MeOH
BIND SAMPLE	Aspirate/ dispense using XTR tips with SCX sorbent x5
WASH 1	Aspirate/ dispense 0.1 N HCl x2
WASH 2	Aspirate/ dispense 100% MeOH x2
ELUTE ANALYTES	Aspirate/ dispense DCM/IPA/NH₄OH x3

Pre-Scripted Application for Hamilton Robotics

To order the exact product in this application and duplicate this method use this DPX catalog number.

DPX Product	Sample Volume	Offline Processing	Online Processing
Catalog #: DPX170178 XTR tips with 5 mg SCX (60 µm) in 1 mL Hamilton	100 µL	Solution Prep Solvent Evaporation	< 15 minutes

To customize this product for a varying sample volumes, choose sorbent amount and tip format from available options.

About XTR Tips

Unlike traditional SPE, XTR tips utilize a patented Dispersive Pipette XTRaction technology. Sorbent chemistry is loosely contained within pipette tips; during aspirate and dispense cycles, the disperser actively mixes sample and sorbent, increasing interaction efficiency.

The result: