A Sensitive Liquid Chromatography–Tandem Mass Spectrometry Method for the Measurement of 11β-MNT and 11β-MNTDC in Human Serum
-Summary of MSACL 2017 Poster Presentation by: Feng Bai, Vignesh Iyer, Ronald Swerdloff, and Christina Wang; Los Angeles Biomedical Research Institute at Harbor-UCLA Medical Center
11-beta-methyl-19-nortestosterone (11β-MNT) is the active androgen derived from 11-beta-methyl nortestosterone dodecylcarbonate (11β-MNTDC). 11β-MNT is being developed as a candidate drug of male hormonal contraception. 11β-MNT like dimethandronlone (DMA) binds to both the androgen and progesterone receptor and may provide the suppression of spermatogenesis with one compound instead of two agents, testosterone and a progestin. The objective of this study was to develop and validate a highly sensitive LC-MS/MS method for the simultaneous detection of serum 11β-MNT and 11β-MNTDC levels for research and clinical purposes in men.
| Protein Precipitation | Serum Sample + I.S. + ACN Vortex & Centrifuge Transfer supernatant |
|---|---|
| Condition | Aspirate and Dispense XTR Tips with HybridSPE sorbent chemistry with 500 μL acetonitrile |
| Extraction | Aspirate and dispense the supernatant 2x to bind interferents to sorbent Transfer remaining clean sample to a new glass vial |
| Prep for injection | Dry down the extracted samples Reconstitute with 40% MeOH in 0.1% formic acid H2O for the analysis |
Download the DPX application note for a full summary of the poster presentation.
About XTR Tips
XTR tips utilize a patented Dispersive Pipette XTRaction technology and is the flagship product of DPX. XTR tips can be used for cleanup or bind-wash-elute protocols for a wide variety of sample preparation applications.
Dispersive Pipette XTRaction supports:
- Better quality data
- Fewer repeat batches
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XTR tips can contain loose HybridSPE sorbent material from Millipore Sigma in 30 mg or 50 mg depending on the tip format. You can order XTR tips with this chemistry directly from Millipore Sigma.
