A Sensitive Non-evaporative Extraction of Drugs of Abuse in Urine with HLB

Automation in this Method: Hamilton Robotics

This new approach to the traditional solid phase extraction of therapeutic and abused drugs cuts down on sample volume, standard costs, and time. A XTR tip with 3 mg of Supel™ Swift HLB is utilized to extract 39 compounds across a large range of polarities. A non-evaporative and highly sensitive extraction method for drug monitoring in urine is presented herein. The method provides an overall 10x dilution factor. A small urine volume of 50 μL minimizes the usage of beta-glucuronidase and internal standard. 96 samples are extracted in 15 minutes.

A total of 39 compounds were analyzed. Linear correlation coefficients for all analytes ranged from 0.995-1.00. Percent recoveries at 15 ng/mL ranged from 70-100% for most analytes measured. Matrix effects were all between -31% to 21%; in this case, a negative value represents ion suppression while positive is ion enhancement. All analytes were quantified below the commonly used confirmation cutoff. HLB is a versatile and robust sorbent for extracting a large range of polarities. The use of DPX XTR HLB Tips for the analysis
of drugs of abuse in urine is reproducible, sensitive, and fast. This method provides relevant LOQ’s for a high throughput and fast turnaround laboratory. This method provides optimal efficiency for drug monitoring.

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About XTR Tips

XTR tips utilize a patented Dispersive Pipette XTRaction technology and is the flagship product of DPX. XTR tips can be used for cleanup or bind-wash-elute protocols for a wide variety of sample preparation applications.

Dispersive Pipette XTRaction supports:

  • Better quality data
  • Fewer repeat batches
  • Scale production
  • Low sample volumes
  • Less internal standard, solvent, enzyme, evaporation time & instrument maintenance

XTR tips have been used for extraction and cleanup methods for clinical applications and forensic toxicology.