Introduction

DNA isolation by silica binding is a robust and efficient technique used to recover high-quality genomic DNA from complex biological samples. Magnetic silica bead technology has been widely adopted for these extractions; however, bead capture time, bead carryover, bead resuspension and requirements for magnets on
automation platforms complicate the process. DPX Technologies has developed a novel MicroPorous Xtraction (MPX) Technology that is incorporated into a pipette tip and eliminates magnetic beads and magnets for a fully automated, high throughput solution. This method uses the Hamilton® Robotics platform and a
1 mL MPX Tip from DPX Technologies for rapid bind, wash and elution steps. The MPX technology design and automated method minimizes cross-contamination and enhances reproducibility.

• DNA extraction from 250 μL of whole blood using the 1 mL MPX Tip was compared to the leading bead-based and column-based technologies using their protocols.
• DNA yield (ng/μL), purity (260/280 ratio), and overall quality (gel electrophoresis) were compared.
• The MPX method can process 96 samples from lysate to purified DNA in only 18 minutes and obtained higher yields of DNA with similar or better purities.

Workflow:

1. Lyse
2. Precipitate
3. Bind
4. Wash 1
5. Wash 2
6. Elute

Download the application for the more information about the workflow and results of this method.

A revolutionary technology that incorporates a variety of microporous media options for high efficiency, automated workflows. Replace spin columns or magnetic bead applications with MPX workflows for DNA purification, sub-micron filtration and more!