Automated Desalting and Buffer Exchange of Human IgG Protein in 6M Guanidine

HIGHLIGHTS: Process 96 samples in <15 minutes

Purpose and Objectives

  • Demonstrate the utility of INTip Size Exclusion Chromatography (SEC) for the purification of Human IgG
    protein from 6M guanidine.
  • Recover greater than 80% of IgG with undetectable guanidine in final elution with a 1:1 ratio of sample to
    elution volume.
  • Show consistent column swelling resulting in low % RSD values.
  • Demonstrate the benefits of patent-pending INTip Swelling and how the technology simplifies SEC workflows.

About SEC Tips

SEC tips incorporate a unique pipette tip design for automated group separation of complex sample mixtures and patent-pending INTip Swelling method.

Learn more about SEC tips used in this method.