MicroPorous Xtraction for Analysis of Carboxy-THC in Urine
Automation Compatibility: Hamilton Robotics
This method utilizes the MicroPorous Xtraction (MPX) device with a C18 disk chemistry for solid phase extraction of 11-nor-9- carboxy-delta-9-tetrahydrocannabinol (C-THC) from urine. The MPX device can be integrated with vacuum systems, and allows for processing of samples with low volumes (50 μL) or high volumes (1 mL or more). A low volume injection was used in this method to readily achieve the necessary sensitivity for pre-employment and workplace drug testing and DUID analysis.
The recovery of C-THC in urine was 81% for the highest concentration tested of 1000 ng/mL, even though the solution pH was not adjusted from the neutral pH hydrolysis conditions. In this method, the calibration plot was linear from 5 ng/mL to 1,000 ng/mL with a linear regression of 0.999.
MPX with C18 can be used to provide accurate quantitation of C-THC rapidly from urine with LC-MS/ MS analysis. This method presented eliminates addition of acid, extracts samples in a rapid and automated fashion (up to 96 samples in less than 15 minutes), and provides purified extracts to maximize LC-MS/MS cleanliness. Download the application note to see the full method and results.
About MPX Technology
The MPX device utilizes a novel design that combines various microporous media with the benefits of automation for improved sample preparation.
Technology Advantages:
- DNA purification or cleanup
- Small molecule extraction
- Sub-micron filtration