MicroPorous Xtraction Technology (MPX)

A revolutionary technology that incorporates a variety of microporous media options for high efficiency workflows for sample preparation.

MPX Device Anatomy

The MPX device utilizes a novel design that combines various microporous media with the benefits of automation for improved sample preparation. The microporous media provides high surface areas required to replace existing large bed SPE, spin column and magnetic bead formats. The device comes as a 3–part unit comprised of an adaptor for Hamilton robotic platforms, a needle support, and the microporous media.

Technology Advantages:

  • DNA purification or cleanup
  • Small molecule extraction
  • Sub-micron filtration
  • Protein isolation or removal- coming soon!

Patent-Pending, innovative workflows that revolutionize and automate a variety of applications

While the MPX device has a reservoir of ~200 μL, the customized vacuum process and high capacity media allow for multiple loading cycles which results in potential large concentration factors. Unlike most vacuum workflows the MPX device and methods allow for fast vacuum processing while maintaining the use of the automated liquid handler for an on-deck elution step. This prevents the hassle and time of removing a manifold throughout the process as well as eliminates concerns of cross contamination. Vacuum blocks are custom designed for MPX methods on a Hamilton Nimbus or Hamilton Star system (sold separately).
MPX device and funnel plate loaded onto vacuum block. Funnel plate eliminates risk of cross contamination for robot head to engage device. Vacuum pulls solutions through for multiple loading cycles.
Robot head directly engages the MPX devices after the vacuum step. Elution is dispensed directly into the final well plate.

On-Deck Vacuum-based Workflows

Tip-on-Device Workflow

Tip-on-device for Filtration

Aspirate Sample in 1 mL tip

Pickup MPX Device

Dispense clean sample through MPX Device

Top tip with Gasket creates air tight seal

Particulates captured on filtration media

MPX
Device

These methods incorporate various media at various porosities for high efficiency filtration. Sample volumes can be up to 1 mL and filtration porosity as low as 0.22 um.

MPX Technology on Hamilton Robotics

This video shows the MPX Kit which include the device and accompanying buffers for nucleic acid extraction from whole blood.

 

Chemistry Options and Applications

Explore current MPX applications and contact us for custom method development.

MPX device and accompanying buffers are sold in a matrix specific kit. Our current genomic methods with the MPX device incorporate a proprietary silica-based media for automated DNA isolation in less than 15 minutes.

  • High yield, PCR compatible sample
  • Lysis step only 5 minutes + prep time
  • Save time with no magnetic capture incubation steps
  • On-deck elution prevents cross contamination by using the pipetting head of the robotic liquid handler

Download Applications Notes:

MPX methods utilizing the vacuum block allows for drying of SPE media for water-free elution that is more GC-MS compatible. Current applications include C18 SPE disks to extract up to 1 mL of urine for small molecule analysis by GC-MS.

  • Larger volume sample extractions
  • Enables SPE media drying
  • Low dead volume for high recovery and low volume elutions

MPX Filtration kits include 1 mL top tips and MPX device. MPX methods utilizing the vacuum block can support filtration for large sample volumes for viscous matrices. Contact us for more information on if MPX can support your workflow.

  • 0.22 µm filtration
  • 0.45 µm filtration
  • 1.0 µm filtration

Contact us if you would like to learn more about microporous media for protein removal and purification methods.

MPX Device is sold in a rack of 96 with kit components needed for specific applications.

Compatibility:

Hamilton Robotics Systems

Funnel plate included in MPX kits. Custom vacuum block sold separately.

Compatibility:

Hamilton Nimbus or STAR system

Download our product ordering guide to see what applications we have readily available.

Learn more

Evaluate MPX technology with the chemistry you need. Contact us to learn more.