This webinar explores different strategies for sample preparation of serum for mass spectrometry analysis. We will provide method recommendations suited to sample size, analyte polarity, dilution factors, etc for a variety of applications using serum, whole blood or dried blood spots as the matrix. Automated INTip Filtration can be applied to replace offline, labor intensive centrifugation steps.

Agenda:

• About DPX
• Strategy 1: Dilute Serum followed by Microelution SPE with µXTR tips
  • µXTR chemistry options and tip styles
• Strategy 2: “Soft Protein Precipitation” with ZnSo₄ followed by ToT Filtration
  • LC-MS/MS Quantification of Testosterone in Serum
  • Sensitive LC-MS/MS Analysis of Therapeutic and Abused Drugs in Whole Blood
• Strategy 3: Full Protein Precipitation with Tip-on-Tip followed by cleanup
  • HybridSPE® XTR tips for phospholipid removal
  • DPX + SALLE