INTip Filtration utilizes a patent-pending Tip-on-Tip Technology that combines a top wide bore conductive tip with a bottom Filtration tip.

1. ToT Filtration Method

ToT Filtration methods provide a fast, automated INTip™ alternative to filtration/centrifugation. This methodology is ideal for high-throughput automated protein precipitation and filtration, beta-glucuronidase enzyme removal, or any other particulate removal, like cannabis solvent extract filtration.

Advantages:

  • No positive pressure/vacuum manifold
  • Low Cost
  • High throughput – fast methods
  • Automated alternative to manual centrifugation steps

Click the lightbox image for full schematic.

Introduce chemistry for additional cleanup.

Tip-on-Tip Filtration can be modified to incorporate resin for more extensive purification. These workflows can center around binding matrix to the resin (Tip-on-Tip Cleanup) where the filtrate has purified analyte to be further analyzed or around binding the analyte (ToT SPE) to be further washed and eluted . In each workflow, the resin and sample solution are mixed in a well for binding to occur. The top tip aspirates the resin/sample mixture, fits into the Filtration tip, and dispenses through the Filtration tip where the resin is retained, as seen in the image provided. These workflows are compatible with a variety of commercially available resins.

ToT Cleanup or SPE methodologies are ideal for applications that require specific incubation conditions between resin and sample solution, like immunoaffinity purification. This provides an automated, cost effective alternative to workflows that traditionally use magnetic beads. Filtration tips are compatible with budget friendly polymer- and agarose-based resins; designed to eliminate particulates in the elution and maximize the utility of the automated liquid handling system.

2. ToT Cleanup Method

Advantages:

  • Cleanup for viscous matrices like cell lysates
  • Compatible with a variety of chemistries
  • Customer can provide their own resin

Click the lightbox image for full schematic.

3. ToT SPE Method

Advantages:

  • Customer can provide their own resin
  • Completely customizable
  • SPE for viscous sample matrices
  • Ideal for nanoparticle and antibody-conjugated drug formulation analyses

Click the lightbox image for full schematic.

Filtration Pipette Tips for ToT Methods:

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dual phase filtration tip
Two filters in series provide maximum filtration without clogging for higher volume blood and serum protein precipitation applications.
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High efficiency sub-micron matrix filtration including urine, blood, and serum.
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Minimal dead volume ideal for small volume blood and serum protein precipitation applications.
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Disperser allows optimal solid phase extraction conditions for Filtration SPE methods. Two frit options are available for varying particle sizes.

Applications:

automated protein precipitation with Tip-on-Tip and Integra pipettes
automated quantitation of testosterone from serum
Urine Filtration to replace manual centrifugation step

Clinical Laboratory Case Study

“The Tip-on-Tip extraction performed using DPX Dual Phase Filtration Tips allows us to achieve the sensitivity we need, while providing a cleaner sample for our mass spec.”

Cannabis Laboratory Case Study

“The DPX Filtration tips greatly simplified our workflow and our analysts were extremely happy with the change. Our stock of other filtration products were much more labor intensive.”