Tip-on-Tip Filtration can be modified to incorporate resin for more extensive purification. These workflows can center around binding matrix to the resin (Tip-on-Tip Cleanup) where the filtrate has purified analyte to be further analyzed or around binding the analyte (ToT SPE) to be further washed and eluted . In each workflow, the resin and sample solution are mixed in a well for binding to occur. The top tip aspirates the resin/sample mixture, fits into the Filtration tip, and dispenses through the Filtration tip where the resin is retained, as seen in the image provided. These workflows are compatible with a variety of commercially available resins.
ToT Cleanup or SPE methodologies are ideal for applications that require specific incubation conditions between resin and sample solution, like immunoaffinity purification. This provides an automated, cost effective alternative to workflows that traditionally use magnetic beads. Filtration tips are compatible with budget friendly polymer- and agarose-based resins; designed to eliminate particulates in the elution and maximize the utility of the automated liquid handling system.