Sample Preparation for Blood/Serum Matrices

Webinar On-demand

This webinar explores different strategies for sample preparation of serum for mass spectrometry analysis. We will provide method recommendations suited to sample size, analyte polarity, dilution factors, etc for a variety of applications using serum, whole blood or dried blood spots as the matrix. Automated INTip Filtration can be applied to replace offline, labor intensive centrifugation steps.

Agenda:

  • About DPX
  • Strategy 1: Dilute Serum followed by Microelution SPE with µXTR tips
    • µXTR chemistry options and tip styles
  • Strategy 2: “Soft Protein Precipitation” with ZnSo4 followed by ToT Filtration
    • LC-MS/MS Quantification of Testosterone in Serum
    • Sensitive LC-MS/MS Analysis of Therapeutic and Abused Drugs in Whole Blood
  • Strategy 3: Full Protein Precipitation with Tip-on-Tip followed by cleanup
    • HybridSPE® XTR tips for phospholipid removal
    • DPX + SALLE

Checkout the other webinars in this series

  1. INTip SPE – Walk away from your sample preparation
  2. Dispersive Pipette Xtraction + Salt Assisted Liquid-Liquid Extraction (DPX + SALLE)
  3. Automated INTip Filtration– replace 96-well filter plates