The analysis of biological samples is an interdisciplinary task including therapeutic drug monitoring, pharmacokinetic studies, clinical analysis, drug metabolism and forensic analysis. Many biological fluids are not suitable for direct liquid chromatography-mass spectrometry analysis due to high content of interfering compounds with target analytes often present in low concentrations. Therefore, sample preparation is often required for bioanalytical methods.

Explore the different topics below and sign up for 1 or all 4!

Sample Preparation for Blood or Serum Matrices:

On-deck Solutions: Using INTip Technology to Increase Throughput while Improving Robustness and Efficiency

On-Demand Webinar Coming Soon

Automated INTip Salt-assisted Liquid-Liquid Extraction for Easy Cleanup

On-Demand Webinar Coming Soon
Intro INTip SPE:

Walk Away from Your Sample Preparation

Watch On-demand
Intro INTip Filtration:

Simplified Filtration with Automated
Tip-on-Tip Technology

On-Demand Webinar Coming Soon

Topic Descriptions and Agenda:

This webinar explores different strategies for sample preparation of serum for mass spectrometry analysis. We will provide method recommendations suited to sample size, analyte polarity, dilution factors, etc for a variety of applications using serum, whole blood or dried blood spots as the matrix. Automated INTip Filtration can be applied to replace offline, labor intensive centrifugation steps.


  • About DPX
  • Strategy 1: Dilute Serum followed by Microelution SPE with µXTR tips
    • µXTR chemistry options and tip styles
  • Strategy 2: “Soft Protein Precipitation” with ZnSo4 followed by ToT Filtration
    • LC-MS/MS Quantification of Testosterone in Serum
    • Sensitive LC-MS/MS Analysis of Therapeutic and Abused Drugs in Whole Blood
  • Strategy 3: Full Protein Precipitation with Tip-on-Tip followed by cleanup
    • HybridSPE® XTR tips for phospholipid removal
    • DPX + SALLE

Combine Dispersive Solid Phase XTRaction + salt-assisted Liquid-Liquid Extraction for a novel cleanup mechanism. XTR tips can house sorbents like weak anion exchange (WAX) or reverse phase (RP) for cleanup and the salt (S) necessary for SALLE. This methodology is ideal for applications that require acetonitrile for protein precipitation and are focused on hydrophobic analytes. This webinar will provide a background of the methodology and specific details on a few applications of interest.


  • INTip SPE Overview
  • Automation Compatibility
  • What is INTip Salt-assisted Liquid-liquid Extraction?
    • Benefits
    • Generic Workflow
    • Pros/Cons
  • Small Molecule Applications
    • LC-MS/MS Analysis of THC & Metabolites in Urine
    • LC-MS/MS Analysis of Cortisol in Urine and Saliva
  • How to Order

Watch this webinar on-demand!

ToT Technology combines a top wide bore conductive tip with a bottom filtration tip to provide an automated alternative to traditional filtration/ centrifugation steps. This methodology is ideal for high throughput protein precipitation, beta-glucuronidase enzyme removal, or any other particulate removal. Sorbent chemistry can be incorporated for a more extensive purification with ToT Cleanup and SPE methods. This webinar will explore the benefits of using Filtration tips and ToT methods in place of syringe filters, vial filters or some filter plates.


  • About DPX
  • Tip-on-Tip Overview & Anatomy
    • Replace 96-well Filter Plates
  • Methods
    • Automation
    • Simple Filtration Workflows
    • Cannabis Filtration
    • ToT Cleanup Workflow
    • ToT SPE Workflow
  • How to Order