2021 Webinar Series On-Demand
The analysis of biological samples is an interdisciplinary task including therapeutic drug monitoring, pharmacokinetic studies, clinical analysis, drug metabolism and forensic analysis. Many biological fluids are not suitable for direct liquid chromatography-mass spectrometry analysis due to high content of interfering compounds with target analytes often present in low concentrations. Therefore, sample preparation is often required for bioanalytical methods.
Explore DPX’s variety of solutions or sample preparations by viewing the webinars below.
Topic Descriptions and Agenda:
Learn how to fully automate methods with INTip SPE. This webinar will explore the benefits of switching from a traditional SPE product like a column or 96 well-plate to a tip-based sample preparation method for solid phase extraction protocols. INTip SPE utilizes a patented technology known as Dispersive Pipette XTRaction. This device is unique from all other SPE devices because sorbent is loosely contained within a pipette tip. Our methods achieve high sensitivity, even for low sample volumes and low concentration of the analyte.
Agenda:
- About DPX
- What is INTip SPE?
- Benefits over Traditional SPE
- Automation Compatibility
- Chemistry Options and Tip Formats
- NEW HLB Sorbent
- HybridSPE – phospholipid removal
- Small Molecule Applications
- LC-MS/MS Analysis of Catecholamines in Urine
- LC-MS/MS Analysis of Fentanyl & Buprenorphine in Urine
- Custom Method Development
- How to Order
Watch this webinar on-demand!
ToT Technology combines a top wide bore conductive tip with a bottom filtration tip to provide an automated alternative to traditional filtration/ centrifugation steps. This methodology is ideal for high throughput protein precipitation, beta-glucuronidase enzyme removal, or any other particulate removal. Sorbent chemistry can be incorporated for a more extensive purification with ToT Cleanup and SPE methods. This webinar will explore the benefits of using Filtration tips and ToT methods in place of syringe filters, vial filters or some filter plates.
Agenda:
- About DPX
- Tip-on-Tip Overview & Anatomy
- Replace 96-well Filter Plates
- Methods
- Automation
- Simple Filtration Workflows
- Cannabis Filtration
- ToT Cleanup Workflow
- ToT SPE Workflow
- How to Order
Combine Dispersive Solid Phase XTRaction + salt-assisted Liquid-Liquid Extraction for a novel cleanup mechanism. XTR tips can house sorbents like weak anion exchange (WAX) or reverse phase (RP) for cleanup and the salt (S) necessary for SALLE. This methodology is ideal for applications that require acetonitrile for protein precipitation and are focused on hydrophobic analytes. This webinar will provide a background of the methodology and specific details on a few applications of interest.
Agenda:
- INTip SPE Overview
- Automation Compatibility
- What is INTip Salt-assisted Liquid-liquid Extraction?
- Benefits
- Generic Workflow
- Pros/Cons
- Small Molecule Applications
- LC-MS/MS Analysis of THC & Metabolites in Urine
- LC-MS/MS Analysis of Cortisol in Urine and Saliva
- How to Order
This webinar explores different strategies for sample preparation of serum for mass spectrometry analysis. We will provide method recommendations suited to sample size, analyte polarity, dilution factors, etc for a variety of applications using serum, whole blood or dried blood spots as the matrix. Automated INTip Filtration can be applied to replace offline, labor intensive centrifugation steps.
Agenda:
- About DPX
- Strategy 1: Dilute Serum followed by Microelution SPE with µXTR tips
- µXTR chemistry options and tip styles
- Strategy 2: “Soft Protein Precipitation” with ZnSo4 followed by ToT Filtration
- LC-MS/MS Quantification of Testosterone in Serum
- Sensitive LC-MS/MS Analysis of Therapeutic and Abused Drugs in Whole Blood
- Strategy 3: Full Protein Precipitation with Tip-on-Tip followed by cleanup
- HybridSPE® XTR tips for phospholipid removal
- DPX + SALLE
