Do you need Solid Phase Extraction for Phospholipid Removal?
A simple protein precipitation or liquid-liquid extraction method may fail to address the problem of phospholipid-induced ion suppression.
Phospholipids are present as a major component of all cell membranes. Therefore, they are present in all biological sample matrices including serum, plasma and whole blood. Phospholipids can pose issues when co-eluting with analytes of interest via LC-MS analysis of small molecules. This co-elution results in ion suppression of the mass spec signal that can cause variability and impact LC-MS result accuracy. Even if the phospholipids do not co-elute with the analyte of interest, they can accumulate on the LC column and elute sporadically in downstream analyses. This can cause unpredictabile ion suppression resulting in poor reproducibility and potentially effect accuracy. (1)
HybridSPE is a unique chemistry offered in a pipette tip for Dispersive Pipette XTRaction as a simple methodology to remove phospholipids. For an automated INTip protein precipitation protocol start with our Tip-on-Tip method and then use HybridSPE XTR tips for extraction prior to analysis.